anti-D-Tag Antibody

DYKDDDDK Tag

D-Tag, anti-D-Tag, D-Tag antibody,D-Tag antibody

DYKDDDDK Tag antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems

Rabbit

Polyclonal

Rabbit IgG

All species

A synthetic D- tag peptide DYKDDDDK conjμgated to KLH.

0.5mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-D-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.

Affinity purified

Western blot

The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps