anti-RFP (dsRed) Antibody

RFP (dsRed)

Red Fluorescent Protein

Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Rabbit

Polyclonal

Rabbit IgG

All species

Recombinant RFP expressed from E. coli

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-RFP polyclonall antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). Based on researchers’ feedback, it can also be used in immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps