anti-Red Fluorescent Protein (dsRed) Antibody

RFP (dsRed)

Red Fluorescent Protein

Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Mouse

Monoclonal

Mouse IgG2b

BV3-2

All species

Recombinant Red Fluorescent Protein (dsRed) expressed from E. coli (Cat.# 4997-100)

0.5 mg/ml

Liquid

100 μg (0.5 mg/ml) Protein A affinity purified mouse anti-dsRed monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot, Immunofluorescence, Immunohistochemistry

The antibody can be used for Western blotting (1-4 μg/ml), immunofluorescence (10-20 μg/ml) and immunohistochemistry (10-20 μg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

red dye

anticorps