anti-HA-Biotin Antibody (Clone 2C16)

HA-Tag-Biotin

GRIN2A , hNR2A , NMDAR2A , NR2A

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Mouse

Monoclonal

Mouse IgG

2C16

All species

Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified anti-HA-Biotin monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunofluorescence

Western blot analysis, immunoprecipitation, and localization of recombinant HA-tagged proteins by standard immuno detection methods (2-10 µg/ml) using Avidin-FITC or other tags. However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

anticorps