anti-His-Tag Antibody

His-Tag

His tag, HHHHHH tag

A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Rabbit

Polyclonal

Rabbit IgG

All species

6xHis synthetic peptide

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation/IC

Western blotting (1 µg/ml), immunoprecipitation (40 µg/ml), and immunocytochemistry (2 µg/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

histidine

anticorps