anti-GFP Antibody

Catalog number
3999-100
Name
anti-GFP Antibody
Size
GFP Antibody
Price
338.00 EUR
Supplier

Details

Target of this antibody:
GFP
Alternative names of antibody target:
Green Fluorescent Protein
Background information:
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
All species
Immunogene sequence:
Recombinant EGFP
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Recommended usage:
Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP (Cat.# 4997-100), YFP (Cat.# 4998-100) and CFP (Cat.# 4996-100) can be used as positive controls.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps