anti-APP Antibody (CT)

APP

amyloid-b-peptide, amyloid beta peptide, Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, Cerebral vascular amyloid peptide,

Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.

Rabbit

Polyclonal

Rabbit IgG1

Human, mouse, rat

A peptide corresponding to the amino acids 737 to 751 of APP or 85 to 99 of the C99 fragment generated by b-secretase cleavage.

1 mg/ml

Liquid

100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.

Affinity chromatography purified via peptide column.

Western blot, Immunohistochemistry, ELISA

Immunohistochemistry: 2 μg/ml; Western Blot: 1 – 2 μg/ml; ELISA. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps