anti-ATG16 Antibody (Center)

ATG16

Autophagy protein 16, Autophagy related protein 16, ATG16L, APG16

Autophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) throμgh phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjμgate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been sμggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Rabbit

Polyclonal

Rabbit IgG1

Human, mouse, rat

ATG16 antibody was raised against a 18 amino acid peptide from near the center of human ATG16.

1 mg/ml

Liquid

100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.

Affinity chromatography purified via peptide column.

Western blot, Immunocytochemistry, ELISA

Western Blot: 0.25 – 0.5 μg/ml, Immunocytochemistry: 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps