anti-LAMP-2 Antibody (CT)

LAMP-2

LAMP-2, Lysosome associated membrane protein 2

Autophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.

Rabbit

Polyclonal

Rabbit IgG1

Human, mouse

LAMP-2 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human LAMP-2.

1 mg/ml

Liquid

100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.

Affinity chromatography purified via peptide column.

Western blot, Immunocytochemistry, ELISA

Western Blot: 1 - 2 μg/ml, Immunocytochemistry: 10 μg/ml, ELISA. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps