anti-IL-33 Antibody

IL-33

NF-HEV

Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.

Rabbit

Polyclonal

Rabbit IgG

Human

E.coli derived Recombinant Human IL-33

N/A

Liquid

A sterile filtered antibody solution in PBS, pH 7.2.

Affinity chromatography

Western blot, ELISA, Neutralization, Immunohistochemistry

1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution) 3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.4) Immunohistochemistry: 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps