anti-KLF4 mAntibody (4G6E11)

KLF4

Kruppel-like factor 4, GKLF, EZF

KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development sμggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

Mouse

Monoclonal

Mouse IgG1

4G6E11

Human, Mouse, Rat

KLF4 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.

1 mg/ml

Liquid

100 µg (1 mg/ml) in 1X PBS containing 0.02 % sodium azide.

Affinity chromatography purified via peptide column

Western blot, ELISA

Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.