anti-mCherry mAntibody (Clone 1)

mCherry

red fluorescent protein mCherry, mCherry red fluorescent protein

mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostabilty. It is an ideal tool for monitoring the dynamics of tagged proteins for protein-protein integration, cell-sorting, organelle labeling, protein localization, gene expression and more. The anti-mCherry antibody recognizes over expressed recombinant proteins containing the mCherry tag fused to either the amino- or carboxy termini of targeted proteins in transfected mammalian cells or other expression systems.

Mouse

Monoclonal

Mouse IgG1

Clone 1

All species

Recombinant mCherry expressed in E. coli (Cat. # 4993-100)

0.5 mg/ml

Liquid

100µg (0.5 mg/ml) in 1X PBS, pH 7.4 with 50% Glycerol

Protein G purified

Western blot

Western blotting (10 µg/ml).

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.