Target of this antibody:
Alternative names of antibody target:
IGFBP-5, IGFBP5, IGFBP 5, IGF-BP-5, IGF-BP 5
Human IGF-BP5 is a cysteine-rich secreted protein produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Studies show that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole body growth inhibition, and retarded muscle development.
Clonality of antibody:
This antibody reacts with:
E. coli expressed human IGF-BP5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-human IGF-BP5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human IGFBP-5 (Cat.# 4723-25) can be used as a positive control. Other applications have not been determined.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.