anti-Wnt-1 Antibody

Wnt-1

wnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1

Wnt-1 is a secreted protein that signals throμgh the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.

Rabbit

Polyclonal

Rabbit IgG

Human

E. coli expressed human Wnt-1

0.5 mg/ml

Liquid

30 µg (0.5 mg/ml) affinity purified rabbit anti-human Wnt-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Wnt-1 (Cat.# 4754-10) can be used as a positive control.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps