anti-HSP70 antibody

HSP70

Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2, HSPA1A, HSPA1, HSPA1B

HSP70 is a class of highly conserved stress proteins. HSP70 is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible HSP70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. HSP70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Therefore, detection of HSP70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.

Rabbit

Polyclonal

Rabbit IgG

Human

E. coli-expressed recombinant human HSP70

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human HSP70 (Cat.# 4859-25) can be used as a positive control

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps