anti-MBD1 (Clone 100B272.1) Antibody

Catalog number
6103-50
Name
anti-MBD1 (Clone 100B272.1) Antibody
Size
MBD1 (Clone 100B272.1) Antibody
Price
295.00 EUR
Supplier

Details

Target of this antibody:
MBD1
Alternative names of antibody target:
Methyl-CpG-Binding Domain 1
Background information:
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD).The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) co-localize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
Clone:
100B272.1
This antibody reacts with:
Human
Immunogene sequence:
Human MBD1 amino acids 391-405
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
50 µg in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide
Purification method:
Affinity chromatography purified via peptide column (protein G)
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blot analysis (2-4 µg/ml). However, the optimal conditions should be determined individually.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps