anti-SIRT7 Antibody

SIRT7

SIR2; Sirtuin 7; Silent Information Regulator 7

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. SIRT7 is a member of this family of proteins and is present in prokaryotes and eukaryotes. All SIR2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast, and mammalian sirtuins are able to metabolize NAD+ and several act as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but as mentioned above, recent reports of histone-activated SIR2-mediated NAD+ metabolism and NAD+-activated SIR2-mediated histone deacetylation sμggest a possible coupled reciprocal activation mechanism involving interactions of SIR2 with NAD+ and the N-ε-acetyl-lysine groups of acetylated histone.

Rabbit

Polyclonal

Rabbit IgG

Human

Human SIRT7 aa 35-51 and 361-377

N/A

Liquid

50 µg of antibody in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide

Protein G purified

Western blot

Western blot: 1-3 µg/ml. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps