anti-DNA Methyltransferase 1 (Clone 60B1220.1) Antibody

DNMT1

DNMT1

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. DNMT1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites sμggesting a link between DNA methylation, histone deacetylase, and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Mouse

Monoclonal

Mouse IgG1κ

60B1220.1

Human, mouse, zebrafish

Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human DNMT1

N/A

Liquid

50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.

Antigen affinity purified

Western blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation

Western blot: 2-4 µg/ml. IHC (parffin-embedded sections), ChIP, and IP: 1-2 µg/ml. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps