PCAF bromodomain (492-658 aa), human recombinant

HAT/PCAF

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.

N/A

N/A

p300/CBP-associated factor; KAT2B

p300/CBP-associated factor; KAT2B

8850

PCAF

Human

Q92831

Yes

E. coli

≥95%

SDS-PAGE, HPLC

N/A

≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)

≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)

≥0.2 U/mg

N/A

One unit of HAT activity is the amount of enzyme that generates 1.0 µmol of CoA per minute at 30°C under kit assay conditions.

-20°C

gel pack

Liquid

30% Glycerol in PBS

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants