anti-USP5 Antibody

USP5

Deubiquitinating enzyme 5, Isopeptidase T, IsoT, ISOT, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin-specific-processing protease 5, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5.

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP5 (ubiquitin specific peptidase 5), also known as ISOT (isopeptidase T), is a 858 amino acid zinc-binding deubiquitinating enzyme that participates in the Ub pathway. A member of the peptidase C19 family, the catalytic activity of USP5 involves a combination of the ubiquitin carboxyl-terminal thiolester and water to produce ubiquitin and a thiol. USP5 contains two UBA domains and one UBP-type zinc finger. USP5 is responsible for disassembling unanchored polyubiquitin chains in the cell. Full length USP5 mediated cleavage of Ub-PLA2 is significantly enhanced is enhanced by low concentrations of mono ubiquitin.

Chicken

Polyclonal

Chicken IgG

Human

Recombinant full length protein

N/A

Liquid

50 µg of antibody in PBS containing 10% glycerol

Purified from egg yolk

Western blot

Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps