anti-EGLN1 Antibody

EGLN1

EGLN1, DKFZp 61F179, ECYT3, HIFPH2, HPH2, PHD2, SM­20, SM20, ZMYND6.

PHD1 (Egln2), PHD­2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle. The mRNA levels of PHD are upregulated by HIF through the hypoxia response element under low oxygen conditions. These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Recombinant protein of human EGLN1

1 mg/ml

Liquid

100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3

Affinity purified

Western blot, Immunohistochemistry

Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps