anti-SMARCA4 Antibody

SMARCA4

BAF190, BRG1, FLJ39786, SNF2, SNF2­BETA, SNF2L4, SNF2LB, SWI2, hSNF2b.

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone­DNA contacts by ATP­dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP­dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM. This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant. SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation. Brg1/hBRM has been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors. Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β. Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

A synthetic peptide of human SMARCA4

1 mg/ml

Liquid

100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3

Affinity purified

Western blot, Immunohistochemistry

Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps