anti-N6-Methyladenosine Antibody

N6-Methyl Adenosine

m6A

N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Rabbit

Polyclonal

Rabbit IgG

Mammalian samples

This antibody is generated from rabbits immunized with a BSA conjugated N6-Methyladenosine (BV-N13-14).

1 mg/ml

Liquid

100 µg (1 mg/ml) in PBS (prepared using DEPC-treated water) with 0.09% (W/V) sodium azide.

Protein A and immunogen affinity purified antibody

Western blot, Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).

Western blot: 1-4 µg/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps