anti-PARP Antibody (Clone 839120)

PARP

PARP1, ADPRT, PPOL, NAD (+) ADP-ribosyltransferase 1

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Mouse

Monoclonal

Mouse IgG1

Human

E. coli-derived recombinant human PARP- Thr 373-Glu 540

0.5 mg/ml

Liquid

100 µl of 0.5 mg/ml antibody in sterile PBS.

Protein A/G Purified Antibody

Western Blot, IHC

WB: 1 µg/ml, IHC: 8-25 µg/ml.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps