anti-Caspase-9 Antibody

Catalog number
6670-100
Name
anti-Caspase-9 Antibody
Size
Caspase-9 Antibody
Price
338.00 EUR
Supplier

Details

Target of this antibody:
Caspase-9
Alternative names of antibody target:
Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6
Background information:
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human
Immunogene sequence:
Recombinant protein of human Caspase-9 (Cat # 1089)
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification method:
Affinity Purified Antibody
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blot: 1-4 µg/ml.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps