anti-OCT4 (OCT3) Antibody

Oct4/Oct3

POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).

N/A

Liquid

Supplied in PBS with 0.09% (W/V) sodium azide.

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot, IF.

Western blot: ~1:1000, IF: ~1:10–1:50.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps