anti-H3K27me3S28p polyclonal antibody

H3K27me3S28p

Histone H3

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

KLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28.

N/A

Liquid

In PBS with 0.05% (W/V) sodium azide.

Whole antiserum from rabbit

Western blot, IF, ELISA, ChIP, Dot blot.

IF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps