anti-H3K9me2 polyclonal antibody

H3K9me2

Histone H3

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, mouse.

KLH-conjugated synthetic peptide of Histone H3 containing dimethylated lysine 9.

N/A

Liquid

In PBS with 0.05% sodium azide and 0.05% ProClin 300.

Affinity purified

Western blot, IF, ELISA, ChIP, Dot blot.

WB: 1:1000, ELISA: 1:1000, Dot Blot: 1:20,000, IF: 1:500, ChIP: 2 µg/ChIP

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps