anti-H4pan polyclonal antibody

H4pan

Histone H4

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a central role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

KLH-conjugated synthetic peptide of Histone H4 containing an unmodified sequence from the central part of the protein.

N/A

Liquid

In PBS with 0.05% sodium azide and 0.05% ProClin 300.

Affinity purified

Western blot, ELISA, ChIP, Dot blot.

ELISA: 1:500, Dot Blot: 1:10,000, ChIP: 2-5 µg/ChIP.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps