anti-RARA polyclonal antibody

RARA

Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1, NR1B1

RARA is a receptor for retinoic acid, a vitamin A metabolite, which directly regulates gene expression in target cells by binding to specific DNA response elements. In the absence of its ligand, this receptor represses transcription through the recruitment of specific corepressors and of HDAC’s, whereas binding of retinoic acid causes the recruitment of coactivators and HAT’s. Translocations involving the RARA gene, often leading to a RARA/PML fusion protein, are a major cause of acute promyelocytic leukemia.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

Human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.

N/A

Liquid

In PBS with 0.05% (W/V) sodium azide.

Whole Anti-Sera

Western blot, ELISA, ChIP.

ChIP: 4 µg/ChIP, WB: 1:750, ELISA: 1:50.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps