anti-MeCP2 polyclonal antibody

MeCP2

AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

MeCP2 is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

MeCP2 (Methyl-CpG-binding domain protein 2) synthetic peptide containing a sequence from the C-terminal conjugated to KLH

N/A

Liquid

In PBS with 0.05% sodium azide and 0.05% ProClin 300.

Affinity Purified

Western blot, ELISA, ChIP.

ChIP: 5 µg/ChIP, WB: 1:1000, ELISA: 1:1000

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps