anti-MBD1 polyclonal antibody

Catalog number
6828-25
Name
anti-MBD1 polyclonal antibody
Size
MBD1 polyclonal antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
MBD1
Alternative names of antibody target:
CXXC3, PCM1, RFT, Methyl-CpG-Binding Domain 1
Background information:
MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC’s) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
Clone:
N/A
This antibody reacts with:
Human.
Immunogene sequence:
MBD1 (Methyl-CpG-binding domain protein 1) synthetic peptide containing a sequence from the N-terminal conjugated to KLH
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Purification method:
Affinity Purified
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA, ChIP.
Recommended usage:
ChIP: 1.5 µg/ChIP, WB: 1:500, ELISA: 1:1000.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps