anti-Runx1/AML1-ETO polyclonal antibody

Runx1/AML1-ETO

AML1-ETO

This antibody specifically recognizes the AML1 (RUNX1) - ETO (RUNX1T1) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

Polyclonal antibody raised in rabbit against the Runx1-ETO fusion protein using a KLH-conjugated synthetic peptide.

N/A

Liquid

In PBS with 0.05% (W/V) sodium azide.

Whole Anti-Sera

Western blot, ELISA, ChIP.

ChIP: 4 µg/ChIP, WB: 1:1000, ELISA: 1:500

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps