anti-Paf1 polyclonal antibody

Paf1

PD2

PAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was first purified from yeast. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. The human PAF (hPAF) complex shares four subunits with yPAF (hCtr9, hPaf1, hLeo1 and Cdc73), but contains an additional higher eukaryotic-specific subunit, hSki8. In addition to coordinating events during transcription (initiation, promoter clearance and elongation); PAF also coordinates events in RNA quality control.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

Polyclonal antibody raised in rabbit against human Paf1 (Paf1, RNA polymerase II associated factor, homolog), using the full length recombinant protein.

N/A

Liquid

In PBS with 0.05% sodium azide and 0.05% ProClin 300.

Protein G purified

Western blot, ChIP.

WB: 1:1000, ChIP: 1-5 µg/ChIP.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps