anti-ETO polyclonal antibody

ETO

RUNX1T1, AML1T1, CBFA2T1, CDR, MTG8, ZMYND2

ETO is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t (8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. This translocation produces a chimeric gene made up of the 5’-region of AML1 and the 3’-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human.

Polyclonal antibody against human ETO using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.

N/A

Liquid

In PBS with 0.05% (W/V) sodium azide.

Whole Anti-Sera

ELISA, ChIP.

ChIP: 4 µl/ChIP, ELISA: 1:100.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps