anti-EzH2 polyclonal antibody

Catalog number
6838-25
Name
anti-EzH2 polyclonal antibody
Size
EzH2 polyclonal antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
EzH2
Alternative names of antibody target:
Enhancer of zeste homolog 2
Background information:
EZH2 is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
Clone:
N/A
This antibody reacts with:
Human, mouse.
Immunogene sequence:
Polyclonal antibody raised in rabbit against the N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Purification method:
Protein G purified
This antibody has been succesfully tested for use in following methods:
Western blot, ChIP, IHC.
Recommended usage:
ChIP: 2.5 µg/ChIP, Western Blot: 1:1000, IHC: 1:100 – 1:500.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps