anti-Setd1b polyclonal antibody

Setd1b

SET1B, KMT2G

SETD1B (UniProt/Swiss-Prot entry Q9UPS6) is a component of the SET1 histone methyltransferase (HMT) complex. This complex specifically methylates lysine 4 of histone H3, but only if the neighboring lysine 9 residue is not yet methylated. Methylation of H3K4 represents a specific tag for epigenetic transcriptional activation. SETD1B shows a non-overlapping localization with SETD1A, suggesting that both proteins play a specific role in the epigenetic control of chromatin structure and gene expression.

Rabbit

Polyclonal

Rabbit IgG

N/A

Mouse.

Polyclonal antibody raised in rabbit against mouse Setd1b (Set domain containing 1B), using a 3 KLH-conjugated synthetic peptides containing sequences from the central part of the protein

N/A

Liquid

In PBS with 0.05% (W/V) sodium azide.

Whole Anti-Sera

Western blot, ELISA.

ELISA: 1:100 – 1:500, Western Blot: 1:500.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Polyclonals and antibodies

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

anticorps