anti-Goat Anti-Rat IgG (H&L) Biotin

Catalog number
6910-250
Name
anti-Goat Anti-Rat IgG (H&L) Biotin
Size
Goat Anti-Rat IgG (H&L) Biotin
Price
295.00 EUR
Supplier

Details

Target of this antibody:
Rat IgG
Alternative names of antibody target:
Anti-Rat IgG, Biotin-Linked Antibody, Biotin linked secondary antibody
Background information:
Goat Polyclonal Secondary Antibody to Rat IgG (H&L) Biotin.
Antibody host:
Goat
Clonality of antibody:
Secondary antibody
Isotype:
Rat IgG
Clone:
N/A
This antibody reacts with:
Rat
Immunogene sequence:
Rat IgG (H&L)
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
In 0.01 M Sodium phosphate, 0.25 M NaCl, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
Purification method:
Affinity Purified
This antibody has been succesfully tested for use in following methods:
IF, FACS
Recommended usage:
ELISA: 1:20,000 – 1:400,000, WB: 1:20,000 – 1:400,000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.
Storage information:
Shelf time: 6 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Latin name
Capra aegagrus hircus,Rattus norvegicus
Additional isotype
Heavy & Light chains,IgG
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.