anti-Goat Anti-Rat IgG (H&L) Biotin

Rat IgG

Anti-Rat IgG, Biotin-Linked Antibody, Biotin linked secondary antibody

Goat Polyclonal Secondary Antibody to Rat IgG (H&L) Biotin.

Goat

Secondary antibody

Rat IgG

N/A

Rat

Rat IgG (H&L)

N/A

Liquid

In 0.01 M Sodium phosphate, 0.25 M NaCl, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.

Affinity Purified

IF, FACS

ELISA: 1:20,000 – 1:400,000, WB: 1:20,000 – 1:400,000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.

Shelf time: 6 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

Capra aegagrus hircus,Rattus norvegicus

Heavy & Light chains,IgG

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.