anti-Goat Anti-Rabbit IgG (H&L) Biotin
Catalog number
6924-100
Name
anti-Goat Anti-Rabbit IgG (H&L) Biotin
Size
Goat Anti-Rabbit IgG (H&L) Biotin
Price
262.00 EUR
Supplier
Details
Target of this antibody:
Rabbit IgG
Alternative names of antibody target:
Anti-rabbit IgG, Biotin-Linked Antibody, Biotin linked secondary antibody
Background information:
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Biotin.
Antibody host:
Goat
Clonality of antibody:
Secondary antibody
Isotype:
Rabbit IgG
Clone:
N/A
This antibody reacts with:
Rabbit
Immunogene sequence:
Rabbit IgG (H&L)
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
Purification method:
Affinity Purified
This antibody has been succesfully tested for use in following methods:
ELISA, WB, IH, IC.
Recommended usage:
ELISA: 1:20000 – 1:400000, WB: 1:20000 – 1:400000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.
Storage information:
Shelf time: 6 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Latin name
Capra aegagrus hircus,Oryctolagus cuniculus
Additional isotype
Heavy & Light chains,IgG
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
