anti-Cyclin E1 Antibody

Cyclin E1

CCNE1, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1

Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, mouse, rat, bovine, dog, pig

This Cyclin E1 antibody is generated from KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1. The exact sequence is proprietary.

1 mg/ml

Liquid

1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen Affinity chromatography

WB, IHC, IF/IC

WB: 1:500 - 1:1000, IHC: 1:100 – 1:200, IF/IC: 1:100 – 1:500

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps