anti-Cyclin F Antibody

Cyclin F

F-box only protein 1, CCNF, FBX1, FBXO1, G2/mitotic-specific cyclin-F

Cyclin F is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. It is ubiquitously expressed in human cells but fluctuates dramatically through the cell cycle, peaking in G2 like cyclin A and decreasing prior to decline of cyclin B. Cyclin F exhibits regulated subcellular localization, being localized in the nucleus in most cells, with a significant percentage of cells showing only perinuclear staining.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, mouse, rat

This Cyclin F antibody is generated from KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin F. The exact sequence is proprietary.

1 mg/ml

Liquid

1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Immunogen Affinity chromatography

WB, IHC

WB: 1:500 - 1:1000, IHC: 1:100 – 1:200.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps