anti-HMGB1 Antibody

Catalog number
A1005-50
Name
anti-HMGB1 Antibody
Size
HMGB1 Antibody
Price
431.00 EUR
Supplier

Details

Target of this antibody:
HMGB1
Alternative names of antibody target:
High mobility group protein B1, High mobility group protein 1, HMG-1, HMG3, SBP-1
Background information:
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
Clone:
N/A
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Recombinant protein of human HMGB1
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
50 µl purified antibody supplied in PBS containing 50% glycerol and 0.02% sodium azide
Purification method:
Affinity purified rabbit IgG
This antibody has been succesfully tested for use in following methods:
Western Blot, Immunohistochemistry, Immunofluorescence
Recommended usage:
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps