anti-Phospho-Bcl-2 (Thr56) antibody

Bcl-2 (Thr56)

BCL2; Apoptosis regulator Bcl-2

The Bcl-2 family of proteins is the best characterized protein family involved in the regulation of apoptotic cell death in variety of cells. Bcl2 is an integral outer mitochondrial membrane protein that regulates mitochondrial calcium homeostasis and proton flux. Several reports suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or S87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human

Synthesized peptide derived from human Bcl-2 around the phosphorylation site of Thr56

1mg/ml

Liquid

100 ug (1mg/ml) of antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Affinity purified rabbit IgG

WB, IHC, IP, IF, ELISA

WB 1:500-1:2000; IHC 1:100-1:300; IP 1:200-500; IF 1:200-1:1000; ELISA 1:20000

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps