anti-Phospho-FoxO1 (Ser319) antibody

FoxO1 (Ser319)

FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma

Forkhead box O (FoxO) transcription factors are downstream targets of the serine/threonine protein kinase B (PKB)/Akt. The Akt kinase regulates processes of cellular proliferation and survival. Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt. The FoxO signaling is regulated by their interactions with other intracellular proteins as well as their post-translational modifications such as phosphorylation. Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, Mouse, Rat

Synthesized peptide derived from human FoxO1 around the phosphorylation site of S319

1mg/ml

Liquid

100 ug (1mg/ml) of antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Affinity purified rabbit IgG

WB, IHC, ELISA

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps