anti-Phospho-Bad (Ser136) antibody

Bad (Ser136)

BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter

Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, Mouse, Rat

Synthesized peptide derived from human Bad around the phosphorylation site of S136

1mg/ml

Liquid

100 ug (1mg/ml) of antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Affinity purified rabbit IgG

WB, IHC, ELISA

WB 1:500-1:2000; IHC 1:100-1:300; ELI+AJ1521+AI1515:AL1533

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps