anti-BCAT1 Monoclonal Antibody

BCAT1

BCAT1, also known as branched chain amino-acid transaminase (cytosolic form), catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. BCAT1 is expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Mouse

Monoclonal

Mouse IgG1, k

AT3C8

Human

Recombinant human BCAT1 (1-386aa) purified from E. coli

1 mg/ml

Liquid

50 µg (1mg/ml) of antibody in PBS, pH 7.4 with 0.02% Sodium Azide, 10% Glycerol

protein-A affinity chromatography

Western blot, ELISA

Western blot 1:1000, ELISA

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

anticorps