TNF-beta, human recombinant

Catalog number

4345-1000

Size

1 mg

Price

3500.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Tumor Necrosis Factor-β, TNFSF1, Lymphotoxin-α (LT-α)

Alternates names

Tumor Necrosis Factor-β, TNFSF1, Lymphotoxin-α (LT-α)

Taglines

A protein that controls proliferation, cellular differentiation, & other functions in most cells

Biomolecule/Target

TGF-beta

NCBI Gene ID #.

4049

NCBI Gene Symbol

TNFB

Gene Source

Human

Accession #

P01374

Recombinant

Yes

Source

E. coli

Purity

≥98%

Activity (Specifications/test method)

N/A

Biological activity

The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of >2 x 10⁷ units/mg.

Molecular Weight

18.6 kDa

Storage Temp.

-20°C

Shipping

Gel pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized protein

Physical form description

Sterile filtered and lyophilized with no additives

Reconstitution Instructions

Reconstitute in PBS to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long term storage, it is recommended to add a carrier protein (0.1% HAS or BSA).

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants

Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.