anti-FSCN1 Monoclonal Antibody

FSCN1

FAN1, HSN, SNL

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells.

Mouse

Monoclonal

Mouse IgG2b, k

AT13D2

Human

Recombinant human FSCN1 (1-493aa) purified from E. coli

1 mg/ml

Liquid

50 µg (1mg/ml) of antibody in PBS, pH 7.4 with 0.02% Sodium Azide, 10% Glycerol

protein-A affinity chromatography

WB, ICC/IF, Flow cytometry, ELISA

WB: 1:1000, ICC/IF: 1:100, Flow cytometry: 2-5ug for 1x106 cells, ELISA

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

anticorps