anti-Anti-human CD9 biotin conjugated antibody

CD9 (biotin conjugated)

DRAP-27 (Diphtheria Toxin Receptor-associated Protein)

Anti-CD9 recognizes a human 24-kiloDalton (kDa) single-chain cell-surface glycoprotein (p24) belonging to the tetraspanin family which transverse the membrane four times. The CD9 antigen has a very broad tissue distribution. It is present on basophils, eosinophils, monocytes, pre-B cells, B cells, and various leukemic cell lines (erythroid, myeloid, some T-lymphoid, pre–B-lymphoid). It is also found on follicular center cells, sinus histocytes, macrophages, Kupffer cells, osteoclasts, hepatocytes, bile duct endothelium, renal glomeruli, proximal and distal tubuli, epithelia (intercellular spinous spaces) of skin and mucosa, fibroblasts, connective tissue, endothelium, smooth muscle, cardiac muscle, synovial lining cells, brain white matter, peripheral nerves and human keratin. In addition to being a co-stimulatory molecule, CD9 antigen activates platelets and induces mitogenesis and kinase activity and is involved in cell migration, cell adhesion, and integrin signaling.

Mouse

Monoclonal

Mouse IgG1

N/A

Human

Pre-B cell line NALM-6

1mg/ml

Liquid

1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM), Approx. pH: 7.4.

Protein A affinity chromatography

WB, ELISA, IHC, IP

WB (1:1000), ELISA (1:500), IHC, IP

Shelf time: Do not use after expiration date stamped on vial label. in temp.: 4 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Biovision supplies antibodies that are for research of human proteins.

Biotinylated

anticorps