PF 4, human platelets

PF 4

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. Human PF-4 a 7.8 kDa protein consisting of 70 amino acid residues.

N/A

7.8 kDa

CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.

CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.

5196

PLF4

Human

P02776

No

Human platelets

≥95%

SDS-PAGE, HPLC

N/A

N/A

Binding of HIT-antibodies.

N/A

N/A

N/A

-20°C

Gel pack

Lyophilized protein

Lyophilized from PBS buffer, pH 7.4.

Reconstitute in sterile ddH₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers.

The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu.

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.